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antibodies against complement factor b cfb  (ABclonal Biotechnology)


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    Structured Review

    ABclonal Biotechnology antibodies against complement factor b cfb
    Antibodies Against Complement Factor B Cfb, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/complement+factor+b+cfb/SERPINA1+Rabbit+pAb/pmc11336080-100-11-28
    Average 93 stars, based on 6 article reviews
    antibodies against complement factor b cfb - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Proteomic analysis of laser captured tubular tissues reveals complement activation and mitochondrial dysfunction in autoimmune related kidney diseases
    Article Snippet: .. The sections were then incubated overnight at 4 °C with primary antibodies against Complement factor B (CFB) and SERPINA1 (rabbit anti-CFB, 1:100, and rabbit anti-SERPINA1, 1:100, both from ABclonal Technology, Wuhan, China). .. After further washing with PBS (3 × 5 min), biotinylated goat anti-rabbit secondary antibody (1:100, Servicebio, Wuhan, China) was applied for 30 min, followed by processing with DAB substrate, according to the manufacturer’s instructions.

    Article Title: An intracellular complement system drives metabolic and proinflammatory reprogramming of vascular fibroblasts in pulmonary hypertension.
    Article Snippet: Subsequently, 30–50 μg of protein was separated by electrophoresis 410 on 4–20% Bis-Tris SDS-PAGE and transferred to a PVDF membrane (Millipore) 411 using a wet transfer system. .. The membrane was blocked with 5% skimmed milk and 412 then incubated with one of the following primary antibodies: anti-C3, 1:5000 413 (ABclonal; A16781), anti-C3a, 1:50 (Hycult Biotech; HM207), anti-CFB, 1:3000 414 (ABclonal; A1706), anti-CFD, 1:3000 (MyBioSource; MBS9203258, Thermo 415 Scientific; PA5-79034), anti-C3aR1, 1:1000 (Santa Cruz; Sc-133172), anti-H3, 416 Page 22 of 51 1:2000 (Cell Signaling Technology; 9715S), anti-α-tubulin, 1:5000 (Sigma; T6199), 417 anti-PDGFRA, 1:3000 (Thermo Scientific; PA534739) and anti-β-Actin 1:10000 418 (Sigma; A5316) for overnight at 4°C, followed by incubated with one of the 419 horseradish peroxidase-conjugated secondary antibodies: anti-rabbit, 1:20000 420 (Invitrogen; 31480) or anti-mouse 1:20000 (Invitrogen; 31430) for 1 hour at room 421 temperature. .. The protein bands were visualized using chemiluminescence 422 (Millipore) and captured on X-ray film.

    other:

    Article Title: Inhibition of HDAC6 alleviates cancer‑induced bone pain by reducing the activation of NLRP3 inflammasome.
    Article Snippet: The following antibodies were used in the cell and tissue analysis: Anti‐glial fibrillary acidic protein (GFAP; cat. no. A19058), anti‐complement factor B (cFB) (cat. no. A13243), anti‐NLRP3 (cat. no. A12694), anti‐NF‐κB (cat. no. A19653), anti‐caspase‐1 (cat. no. A16792), anti‐TNF‐α (cat. no. A11534) and anti‐HdAc6 (cat. no. A1732) were purchased from ABclonal Biotech co., Ltd.; anti‐IL‐1β (cat. no. AF5103), anti‐c3 (cat. no. dF13224), anti‐GFAP (cat. no. BF8023), anti‐NLRP3 (cat. no. BF8029) and anti‐β‐actin (cat. no. AF7018), were purchased from Affinity Biosciences; and anti‐pSer22‐HdAc6 (cat. no. YP0922), which was purchased from ImmunoWay Biotechnology company.

    Tissue:

    Article Title: Inhibition of HDAC6 alleviates cancer-induced bone pain by reducing the activation of NLRP3 inflammasome
    Article Snippet: .. The following antibodies were used in the cell and tissue analysis: Anti-glial fibrillary acidic protein (GFAP; cat. no. A19058), anti-complement factor B (CFB) (cat. no. A13243), anti-NLRP3 (cat. no. A12694), anti-NF-κB (cat. no. A19653), anti-caspase-1 (cat. no. A16792), anti-TNF-α (cat. no. A11534) and anti-HDAC6 (cat. no. A1732) were purchased from ABclonal Biotech Co., Ltd.; anti-IL-1β (cat. no. AF5103), anti-C3 (cat. no. DF13224), anti-GFAP (cat. no. BF8023), anti-NLRP3 (cat. no. BF8029) and anti-β-actin (cat. no. AF7018), were purchased from Affinity Biosciences; and anti-pSer22-HDAC6 (cat. no. YP0922), which was purchased from ImmunoWay Biotechnology Company. .. The secondary antibodies that were used for western blotting were HRP Goat anti-rabbit IgG (H+L; cat. no. AS014) and HRP Goat Anti-Mouse IgG (H+L; cat. no. AS003), which were purchased from ABclonal Biotech Co., Ltd.

    Analysis:

    Article Title: Inhibition of HDAC6 alleviates cancer-induced bone pain by reducing the activation of NLRP3 inflammasome
    Article Snippet: .. The following antibodies were used in the cell and tissue analysis: Anti-glial fibrillary acidic protein (GFAP; cat. no. A19058), anti-complement factor B (CFB) (cat. no. A13243), anti-NLRP3 (cat. no. A12694), anti-NF-κB (cat. no. A19653), anti-caspase-1 (cat. no. A16792), anti-TNF-α (cat. no. A11534) and anti-HDAC6 (cat. no. A1732) were purchased from ABclonal Biotech Co., Ltd.; anti-IL-1β (cat. no. AF5103), anti-C3 (cat. no. DF13224), anti-GFAP (cat. no. BF8023), anti-NLRP3 (cat. no. BF8029) and anti-β-actin (cat. no. AF7018), were purchased from Affinity Biosciences; and anti-pSer22-HDAC6 (cat. no. YP0922), which was purchased from ImmunoWay Biotechnology Company. .. The secondary antibodies that were used for western blotting were HRP Goat anti-rabbit IgG (H+L; cat. no. AS014) and HRP Goat Anti-Mouse IgG (H+L; cat. no. AS003), which were purchased from ABclonal Biotech Co., Ltd.

    Membrane:

    Article Title: An intracellular complement system drives metabolic and proinflammatory reprogramming of vascular fibroblasts in pulmonary hypertension.
    Article Snippet: Subsequently, 30–50 μg of protein was separated by electrophoresis 410 on 4–20% Bis-Tris SDS-PAGE and transferred to a PVDF membrane (Millipore) 411 using a wet transfer system. .. The membrane was blocked with 5% skimmed milk and 412 then incubated with one of the following primary antibodies: anti-C3, 1:5000 413 (ABclonal; A16781), anti-C3a, 1:50 (Hycult Biotech; HM207), anti-CFB, 1:3000 414 (ABclonal; A1706), anti-CFD, 1:3000 (MyBioSource; MBS9203258, Thermo 415 Scientific; PA5-79034), anti-C3aR1, 1:1000 (Santa Cruz; Sc-133172), anti-H3, 416 Page 22 of 51 1:2000 (Cell Signaling Technology; 9715S), anti-α-tubulin, 1:5000 (Sigma; T6199), 417 anti-PDGFRA, 1:3000 (Thermo Scientific; PA534739) and anti-β-Actin 1:10000 418 (Sigma; A5316) for overnight at 4°C, followed by incubated with one of the 419 horseradish peroxidase-conjugated secondary antibodies: anti-rabbit, 1:20000 420 (Invitrogen; 31480) or anti-mouse 1:20000 (Invitrogen; 31430) for 1 hour at room 421 temperature. .. The protein bands were visualized using chemiluminescence 422 (Millipore) and captured on X-ray film.

    Cell-Signaling:

    Article Title: An intracellular complement system drives metabolic and proinflammatory reprogramming of vascular fibroblasts in pulmonary hypertension.
    Article Snippet: Subsequently, 30–50 μg of protein was separated by electrophoresis 410 on 4–20% Bis-Tris SDS-PAGE and transferred to a PVDF membrane (Millipore) 411 using a wet transfer system. .. The membrane was blocked with 5% skimmed milk and 412 then incubated with one of the following primary antibodies: anti-C3, 1:5000 413 (ABclonal; A16781), anti-C3a, 1:50 (Hycult Biotech; HM207), anti-CFB, 1:3000 414 (ABclonal; A1706), anti-CFD, 1:3000 (MyBioSource; MBS9203258, Thermo 415 Scientific; PA5-79034), anti-C3aR1, 1:1000 (Santa Cruz; Sc-133172), anti-H3, 416 Page 22 of 51 1:2000 (Cell Signaling Technology; 9715S), anti-α-tubulin, 1:5000 (Sigma; T6199), 417 anti-PDGFRA, 1:3000 (Thermo Scientific; PA534739) and anti-β-Actin 1:10000 418 (Sigma; A5316) for overnight at 4°C, followed by incubated with one of the 419 horseradish peroxidase-conjugated secondary antibodies: anti-rabbit, 1:20000 420 (Invitrogen; 31480) or anti-mouse 1:20000 (Invitrogen; 31430) for 1 hour at room 421 temperature. .. The protein bands were visualized using chemiluminescence 422 (Millipore) and captured on X-ray film.



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